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qpcrbio cdna synthesis kit  (PCR Biosystems Ltd)


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    Structured Review

    PCR Biosystems Ltd qpcrbio cdna synthesis kit
    Qpcrbio Cdna Synthesis Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 95/100, based on 386 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+reverse+transcriptase+kit/UltraScript+Reverse+Transcriptase/pm42020755-652-16-20
    Average 95 stars, based on 386 article reviews
    qpcrbio cdna synthesis kit - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Integrating image-based phenotyping and QTL mapping to enhance genetic resistance and accelerate breeding for bacterial grain rot resistance in rice.
    Article Snippet: The sampled panicles were used for total RNA extraction following the manufacturer’s instructions for the RNeasy Micro Kit (QIAGEN, Cat. 74,004, Hilden, Germany). .. One microgram of the extracted RNA was used for cDNA synthesis following the manufacturer’s instructions for the qPCRBIO cDNA Synthesis Kit (PCRBIOSYSTEMS, USA). .. The synthesized cDNA was then diluted 1:10 (v/v) in nuclease-free water (QIAGEN, Cat. No. 129114).

    Article Title: TCF25 emerges as a cytoplasmic regulator of GPRASP2 stability
    Article Snippet: .. Briefly, total RNA was extracted using the RNeasy Mini Kit (QIAGEN) and treated with DNase I (Thermo Fisher Scientific) according to the manufacturer’s instructions. cDNA was synthesised from 400 ng intact RNA using the qPCRBIO cDNA Synthesis Kit (PCR Biosystems). .. Quantitative PCR was performed using qPCRBIO SyGreen Blue Mix Lo-ROX (PCR Biosystems) on a QuantStudioTM5 Real-Time PCR System (Thermo Fisher Scientific) using gene-specific primers ( ).

    Article Title: Integrating image-based phenotyping and QTL mapping to enhance genetic resistance and accelerate breeding for bacterial grain rot resistance in rice
    Article Snippet: The sampled panicles were used for total RNA extraction following the manufacturer’s instructions for the RNeasy Micro Kit (QIAGEN, Cat. 74,004, Hilden, Germany). .. One microgram of the extracted RNA was used for cDNA synthesis following the manufacturer’s instructions for the qPCRBIO cDNA Synthesis Kit (PCRBIOSYSTEMS, USA). .. The synthesized cDNA was then diluted 1:10 (v/v) in nuclease-free water (QIAGEN, Cat. No. 129114).

    Article Title: H 2 O 2 repurposes plant O 2 sensing to regulate post-hypoxia responses.
    Article Snippet: RNA concentration was quantified using a NanoDrop ND-1000 (Thermo Scientific), and RNA integrity was tested on a 1% agarose gel. .. One microgram of total RNA was subjected to DNase Treatment (Thermo Scientific) and retrotranscribed using a qPCRBIO cDNA Synthesis Kit (PCR Biosystems). .. Real-time qPCR was performed with a QuantStudio 5 Real-Time PCR System (Applied Biosystems) using Power SYBR Master Mix (Thermo Fisher Scientific).

    Article Title: Sex-specific division of labor in termites is linked to differential brain architecture and foraging gene expression
    Article Snippet: Illustra GFX PCR DNA and Gel Band Purification Kit , Cytiva , Cat#28903470. .. Ultrascript cDNA Synthesis Kit , PCR Biosystems , PB30.11–10. .. qPCRBIO SyGreen Mix Separate-Rox , PCR Biosystems , PB20.14–05.

    Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli .
    Article Snippet: RNA samples were treated with DNase I (Qiagen, Valencia, CA) to eliminate genomic DNA. .. 0.4 μg of total purified RNA was reverse transcribed using a qPCRBIO cDNA synthesis kit (PCR Biosystems, London, UK). .. Quantitative Real-Time PCR (qRT-PCR) was run for 40 cycles on an Eppendorf Mastercycler ep Realplex with a mix of 1 μL (10 ng) cDNA, 1 μL each of forward and reverse primers (10 μM stock), and 10 μL of SyGreen Mix Lo-ROX (PCR Biosystems, London, UK).

    Article Title: Prolyl hydroxylase-dependent proteolysis enables the orthogonal hypoxia responses in plants.
    Article Snippet: .. One microgram of total RNA was treated with DNase (Thermo ScientificTM) to remove genomic DNA before retro-transcription using qPCRBIO cDNA Synthesis Kit (PCR Biosystems). .. Real-time quantitative PCRs were performed in 10 μl volume using a 2X Power SYBRTM Master Mix (Thermo Fisher ScientificTM), 10 ng cDNA and 0.2 μM of specific reverse and complement primers for each gene to be tested.

    Purification:

    Article Title: An innovative in vitro model for studying the biology of cardiac fibroblasts originating from the epicardium.
    Article Snippet: RNA from heart ventricles was isolated using the RNeasy Micro Kit (Qiagen, 74004), following the manufacturer’s instructions. .. The purified RNA was reverse transcribed into cDNA using UltraScript® Reverse Transcriptase (PCR Biosystems, PB30.11-10). .. Relative gene expression levels were quantified using SYBR Master Mix (Promega, A6002); real-time PCR was performed on 96-well QuantStudio 3 plates (Applied Biosystems) using Connect analysis software (Thermo Fisher Scientific).

    Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli .
    Article Snippet: RNA samples were treated with DNase I (Qiagen, Valencia, CA) to eliminate genomic DNA. .. 0.4 μg of total purified RNA was reverse transcribed using a qPCRBIO cDNA synthesis kit (PCR Biosystems, London, UK). .. Quantitative Real-Time PCR (qRT-PCR) was run for 40 cycles on an Eppendorf Mastercycler ep Realplex with a mix of 1 μL (10 ng) cDNA, 1 μL each of forward and reverse primers (10 μM stock), and 10 μL of SyGreen Mix Lo-ROX (PCR Biosystems, London, UK).

    Reverse Transcription:

    Article Title: An innovative in vitro model for studying the biology of cardiac fibroblasts originating from the epicardium.
    Article Snippet: RNA from heart ventricles was isolated using the RNeasy Micro Kit (Qiagen, 74004), following the manufacturer’s instructions. .. The purified RNA was reverse transcribed into cDNA using UltraScript® Reverse Transcriptase (PCR Biosystems, PB30.11-10). .. Relative gene expression levels were quantified using SYBR Master Mix (Promega, A6002); real-time PCR was performed on 96-well QuantStudio 3 plates (Applied Biosystems) using Connect analysis software (Thermo Fisher Scientific).

    Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli .
    Article Snippet: RNA samples were treated with DNase I (Qiagen, Valencia, CA) to eliminate genomic DNA. .. 0.4 μg of total purified RNA was reverse transcribed using a qPCRBIO cDNA synthesis kit (PCR Biosystems, London, UK). .. Quantitative Real-Time PCR (qRT-PCR) was run for 40 cycles on an Eppendorf Mastercycler ep Realplex with a mix of 1 μL (10 ng) cDNA, 1 μL each of forward and reverse primers (10 μM stock), and 10 μL of SyGreen Mix Lo-ROX (PCR Biosystems, London, UK).



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